Journal: Journal of Molecular Medicine (Berlin, Germany)

Article Title: Glis3 as a critical regulator of Pit1-lineages and renal functions

doi: 10.1007/s00109-025-02611-3

Figure Lengend Snippet: Hyperprolactinemia of glis3KD larvae. a Relative mRNA expression of prl CTRL, glis3KD larvae at 120 hpf. qRT-PCR data are normalized to eef1α , and the results are shown as mean ± SD of n = 3 independent replicates. Each data point represents a pool of 20 larvae. Student- t -test was used for statistical analysis between groups: *** P < 0.001. b ELISA of prolactin (ng/ml) in control and glis3KD larvae at 120 hpf. Results (mean ± SD of 3 pools, 50 larvae/pool) were analyzed by Student’s t-test: *** P < 0.001. c , c’ Representative FISH images of prl in CT and KD larvae at 120 hpf. All larvae were acquired by confocal microscopy in ventral view, head to the top. Scale bars in c = 100 µm. d Quantification of total cell volume (µm 3 ) of prl using confocal analysis on the same area (ROI) and number of sections for each embryo. Data are displayed as dot plots with mean ± SD of n = 15 embryos each. Mann–Whitney test was used for statistical analysis, *** P < 0.001. e – f’ IF of prolactin in control and glis3KD larvae in lateral ( e , e’ ) or ventral ( f , f’ ) view. Prl localized in gills (g, asterisks), pectoral fins (pf, arrow), pronephric ducts (pd, arrowheads), forebrain (fb, arrow), and adenohypophysis (ah, arrowhead). Scale bars: e = 300 µm; f = 200 µm. g Mean fluorescence intensity (MFI) of prolactin-positive tissues ( n = 15) using Fiji Software. Mann–Whitney test: ns, not significant; *** P < 0.001

Article Snippet: Prolactin levels (ng/ml) were measured using a Fish PRL ELISA Kit (CusaBio, CSB-E12695Fh).

Techniques: Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Control, Confocal Microscopy, MANN-WHITNEY, Fluorescence, Software

Journal: bioRxiv

Article Title: Prolactin links psychological stress to psoriasis via a fibroblast-chemokine pathway

doi: 10.1101/2025.10.11.681540

Figure Lengend Snippet: a Schematic representation of the cell culture. b Volcano plot of differentially expressed genes between PBS-and PRL-stimulated NIH-3T3 cells ( n = 3). c KEGG pathway enrichment analysis of differentially expressed genes. d GSEA gene enrichment analysis of the chemokine signaling pathway. e Heatmap of chemokines. f The expression of Ccl2, Ccl7, Ccl8 , and Ccl11 as determined by quantitative PCR ( n = 3). g The expression of Ccl2 and Ccl7 in the skin of IMQ-treated female and male mice ( n = 3–4). h GSEA gene enrichment analysis of the JAK-STAT signaling pathway. i Western blot analyses of p-JAK2, JAK2, p-STAT1, STAT1, p-STAT3, STAT3, p-STAT5, and STAT5 in PRL-stimulated NIH-3T3 cells. j Western blot analyses of p-JAK2, JAK2, p-STAT3, and STAT3 as indicated. k The expression of Ccl2 and Ccl7 in PBS-, PRL+Veh-, and PRL+J2S3i-treated NIH-3T3 cells ( n = 3). Data are representative of two ( f, g, i – k ) independent experiments. Data are mean ± SD ( f, g, k ). The P values were calculated by two-tailed unpaired Student’s t -test ( f, g ) or one-way ANOVA with Dunnett’s post-hoc test ( k ); * P < 0.05, ** P < 0.01.

Article Snippet: For RNA-seq, the cells were stimulated with recombinant human PRL (100 ng/mL, MCE, #HY-P71059) for 6 h. For Western blot, the cells were stimulated with different concentrations of PRL (0, 1, 10, and 100 ng/mL) for 30 min, or transfected with siRNA targeting Prlr (5’-UAAAGAAACAGGAAUUGGGTT-3’) or scrambled control for 24 h and then stimulated with PRL (100 ng/mL) for 30 min. For quantitative PCR, the cells were pretreated with JAK2/STAT3 inhibitor FLLL32 (10 μM) for 1 h followed by stimulation with PRL (100 ng/mL) for 6 h.

Techniques: Cell Culture, Expressing, Real-time Polymerase Chain Reaction, Western Blot, Two Tailed Test